Middle East Journal of Rehabilitation and Health

Published by: Kowsar

Evaluation of a New Method for Biological Activity Analysis of Recombinant Human Parathyroid Hormone-Related Protein

Davood Nasrabadi 1 , 2 , 3 , Siamak Rezaeiani 4 , Ali Sayadmanesh 4 , Mohammad Reza Gharaati 4 , Mohamadreza Baghaban Eslaminejad 2 , * and Aliakbar Shabani 1 , **
Authors Information
1 Medical Biotechnology Department, Semnan University of Medical Sciences, Semnan, Iran
2 Department of Stem Cell and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
3 Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran
4 Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Corresponding Authors:
Article information
  • Middle East Journal of Rehabilitation and Health: April 2018, 5 (2); e65828
  • Published Online: April 16, 2018
  • Article Type: Research Article
  • Received: January 2, 2018
  • Revised: February 3, 2018
  • Accepted: February 17, 2018
  • DOI: 10.5812/mejrh.65828

To Cite: Nasrabadi D, Rezaeiani S, Sayadmanesh A, Gharaati M R, Baghaban Eslaminejad M, et al. Evaluation of a New Method for Biological Activity Analysis of Recombinant Human Parathyroid Hormone-Related Protein, Middle East J Rehabil Health Stud. 2018 ; 5(2):e65828. doi: 10.5812/mejrh.65828.

Copyright © 2018, Middle East Journal of Rehabilitation and Health Studies. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Orloff JJ, Reddy D, de Papp AE, Yang KH, Soifer NE, Stewart AF. Parathyroid hormone-related protein as a prohormone: posttranslational processing and receptor interactions. Endocr Rev. 1994;15(1):40-60. doi: 10.1210/edrv-15-1-40. [PubMed: 8156938].
  • 2. Juppner H, Abou-Samra AB, Freeman M, Kong XF, Schipani E, Richards J, et al. A G protein-linked receptor for parathyroid hormone and parathyroid hormone-related peptide. Science. 1991;254(5034):1024-6. doi: 10.1126/science.1658941. [PubMed: 1658941].
  • 3. Stewart AF, Horst R, Deftos LJ, Cadman EC, Lang R, Broadus AE. Biochemical evaluation of patients with cancer-associated hypercalcemia: evidence for humoral and nonhumoral groups. N Engl J Med. 1980;303(24):1377-83. doi: 10.1056/NEJM198012113032401. [PubMed: 6253785].
  • 4. Moseley JM, Kubota M, Diefenbach-Jagger H, Wettenhall RE, Kemp BE, Suva LJ, et al. Parathyroid hormone-related protein purified from a human lung cancer cell line. Proc Natl Acad Sci U S A. 1987;84(14):5048-52. doi: 10.1073/pnas.84.14.5048. [PubMed: 2885845].
  • 5. Mueller MB, Fischer M, Zellner J, Berner A, Dienstknecht T, Kujat R, et al. Effect of parathyroid hormone-related protein in an in vitro hypertrophy model for mesenchymal stem cell chondrogenesis. Int Orthop. 2013;37(5):945-51. doi: 10.1007/s00264-013-1800-1. [PubMed: 23371427].
  • 6. Kim YJ, Kim HJ, Im GI. PTHrP promotes chondrogenesis and suppresses hypertrophy from both bone marrow-derived and adipose tissue-derived MSCs. Biochem Biophys Res Commun. 2008;373(1):104-8. doi: 10.1016/j.bbrc.2008.05.183. [PubMed: 18554504].
  • 7. Lee JM, Im GI. PTHrP isoforms have differing effect on chondrogenic differentiation and hypertrophy of mesenchymal stem cells. Biochem Biophys Res Commun. 2012;421(4):819-24. doi: 10.1016/j.bbrc.2012.04.096. [PubMed: 22554518].
  • 8. Madeddu L, Legros N, Devleeschouwer N, Bosman C, Piccart MJ, LeClercq G. Estrogen receptor status and estradiol sensitivity of MCF-7 cells in exponential growth phase. Eur J Cancer Clin Oncol. 1988;24(3):385-90. doi: 10.1016/S0277-5379(98)90007-4. [PubMed: 3383942].
  • 9. Falzon M, Du P. Enhanced growth of MCF-7 breast cancer cells overexpressing parathyroid hormone-related peptide. Endocrinology. 2000;141(5):1882-92. doi: 10.1210/endo.141.5.7470. [PubMed: 10803599].
  • 10. Birch MA, Carron JA, Scott M, Fraser WD, Gallagher JA. Parathyroid hormone (PTH)/PTH-related protein (PTHrP) receptor expression and mitogenic responses in human breast cancer cell lines. Br J Cancer. 1995;72(1):90-5. doi: 10.1038/bjc.1995.282. [PubMed: 7599071].
  • 11. Torricelli C, Fortino V, Capurro E, Sacchi G, Ponzo P, Pacini A, et al. Role of PTHrp and PTHrp-engaged pathways in MCF-7 cells migration/invasion. Matrix Biol. 2006;25(2):104-11. doi: 10.1016/j.matbio.2005.09.006. [PubMed: 16455237].
  • 12. Solchaga LA, Penick KJ, Welter JF. Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells: tips and tricks. Methods Mol Biol. 2011;698:253-78. doi: 10.1007/978-1-60761-999-4_20. [PubMed: 21431525].
  • 13. Maioli E, Fortino V. PTHrP on MCF-7 breast cancer cells: a growth factor or an antimitogenic peptide? Br J Cancer. 2004;90(6):1293-4. author reply 1295. doi: 10.1038/sj.bjc.6601689. [PubMed: 15026815].
  • 14. Hoey RP, Sanderson C, Iddon J, Brady G, Bundred NJ, Anderson NG. The parathyroid hormone-related protein receptor is expressed in breast cancer bone metastases and promotes autocrine proliferation in breast carcinoma cells. Br J Cancer. 2003;88(4):567-73. doi: 10.1038/sj.bjc.6600757. [PubMed: 12592371].
  • 15. Natarajan K, Berk BC. Crosstalk coregulation mechanisms of G protein-coupled receptors and receptor tyrosine kinases. Methods Mol Biol. 2006;332:51-77. doi: 10.1385/1-59745-048-0:51. [PubMed: 16878685].
  • 16. Bringhurst FR, Juppner H, Guo J, Urena P, Potts JJ, Kronenberg HM, et al. Cloned, stably expressed parathyroid hormone (PTH)/PTH-related peptide receptors activate multiple messenger signals and biological responses in LLC-PK1 kidney cells. Endocrinology. 1993;132(5):2090-8. doi: 10.1210/endo.132.5.8386606. [PubMed: 8386606].
  • 17. Zhang Y, Kumagai K, Saito T. Effect of parathyroid hormone on early chondrogenic differentiation from mesenchymal stem cells. J Orthop Surg Res. 2014;9:68. doi: 10.1186/s13018-014-0068-5. [PubMed: 25079095].
  • 18. Mueller MB, Fischer M, Zellner J, Berner A, Dienstknecht T, Prantl L, et al. Hypertrophy in mesenchymal stem cell chondrogenesis: effect of TGF-beta isoforms and chondrogenic conditioning. Cells Tissues Organs. 2010;192(3):158-66. doi: 10.1159/000313399. [PubMed: 20407224].

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